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How Various Mechanisms By Which Substances Cross

The Cell Membrane Essay, Research Paper


Homeostasis is essential to the cell s survival. The cell membrane is


responsible for homeostasis. The membrane has a selective permeability


which means what moves in and out of the cell is regulated. Amino


acids, sugars, oxygen, sodium, and potassium are examples of substances


that enter the cell. Waste products and carbon dioxide are removed from


the cell. All of these substances cross the membrane in a variety of


ways. From diffusion and osmosis, to active transport the traffic


through the cell membrane is regulated.


Diffusion is the movement of molecules form one area of higher


concentration to an area of lower concentration. Concentration gradient


causes the molecules to move from higher concentration to a lower


concentration. The side of the membrane that has the higher


concentration is said to have the concentration gradient. It drives


diffusion because substances always move down their concentration


gradient. The pressure gradient also plays a role in diffusion. Where


this is a pressure gradient there is motion of molecules. The pressure


gradient is a difference in pressure between two different points. If


the concentration of one side of the membrane is greater than the


molecules will travel from the higher to lower concentration.


Eventually there will be a dynamic equilibrium and there will be no net


movement of molecules from one side to the other.


Osmosis is the diffusion of water. Like diffusion, the water moves


from a region of higher water potential to a region of lower water


potential. Solutions have three different stages that the solutes can


be classified in: isotonic, hypertonic, and hypotonic. Isotonic is when


the solutions have equal amounts of solutes. Like equilibrium, there is


no net change in the amount of water in either solution. When the


solutions have different concentration of solutes then the one with less


solute is hypotonic and the one with more solute is hypertonic.


Hypotonic takes in the solute from the hypertonic side that gives away


the solute. There will be a net movement in these types of solution.


The molecules will move from the hypotonic solution into the hypertonic


solution.


The third way a substance can cross the cell membrane is through


facilitated diffusion. This occurs when special carrier proteins carry


solutes dissolved in the water across the membrane by using active


transport. When the concentration gradient can not allow travel from


one side of the membrane to the other fast enough for the cell s


nutritional needs, then facilitated diffusion is used. The transport


protein is specialized for the solute it is carrying, just as enzymes


are specialized for their substrate. The transport protein can be


changes or blocked just like enzymes. There are several theories on how


proteins facilitate diffusion. In one case t

he transport protein acts


like a revolving door. The protein opens on one end and accepts the


solute and then closes. It opens on the other end and releases the


solute. Another way proteins facilitate diffusion is proteins that


extend over the membrane can provide a shuttle that the selective


solutes may cross. There are proteins where their job is to open and


close a gated channel. Polar molecules and ions blocked by the


phospholipid bilayer diffuse through it by facilitated diffusions.


Intercellular joining is when cell s membranes hook together. Cell to


cell recognition and when different parts of the cytoskeleton bond to


proteins, are both ways that substances may cross the membrane through


facilitated diffusion.


Active transport moves the solutes against their concentration


gradients. Proteins use ATP energy to pump certain substances through


the membrane. One way ATP powers active transport is by a


sodium-potassium pump. The pump exchanges sodium for potassium across


the plasma membrane of animal cells. ATP powers it by transferring a


phosphate group to the protein. Large molecules are transported across


the plasma membrane by vesicles, this is called exocytosis. Vesicles,


which form in the Golgi apparatus, are moved by the cytoskeleton to the


plasma membrane. When the plasma membrane and the vesicle membrane


touch the lipid molecules rearrange themselves so that the two membranes


are fused together. The substance inside the vesicle now overflows


outside of the cell. The opposite method, substances moving into the


cell, is called endocytosis. There are three steps involved in


endocytosis. The first step, phagocytosis, takes in macromolecules and


packages hem membrane-enclosed sac. After the sac fuses with enzymes it


is digested. In the next step, called pinocytosis, the cell engulfs the


sac and its dissolves solute. The final step is receptor-mediated


endocytosis, where only specific substances attach to the receptor


sites. Endocytosis brings substances outside the cell into the cell.


Active transport has one final way of transporting substances across the


cell membrane, and that is called phosphorylation. ATP is made up of


three phosphates that can power active transport by transferring one of


its phosphate groups to a protein. When ATP s phosphate group is


hydrolyzed and the phosphate group is transferred we call it


phosphorylation.


In review, substances cross the cell membrane in two different ways:


passive and active transport. Passive transport includes osmosis,


diffusion, and facilitated diffusion. None of these techniques use


energy. Active transport includes endo and exocytosis, sodium-potassium


pumps. and phosphorylation, all of which expend energy to transport


substances across the cell membrane. The cell has the ability to


regulate what crosses its cellular boundaries.


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